Separate-component-stabilization system for protein and DNA production without the use of antibiotics

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Separate-component-stabilization system for protein and DNA production without the use of antibiotics.

Plasmid instability is a significant concern in the industrial utilization of microorganisms for protein or DNA production. Here we report on the development of a new and highly effective stabilization system based on the use of the ccd antidote/poison genes. For the first time, we separated the antidote gene from the poison gene: localizing the former in the plasmid and integrating the latter ...

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ژورنال

عنوان ژورنال: BioTechniques

سال: 2005

ISSN: 0736-6205,1940-9818

DOI: 10.2144/05385rr02